Laboratory Incubator

An incubator comprises a transparent chamber and the equipment that regulates its temperature, humidity, and ventilation. For years, the principle uses for the controlled environment provided by incubators included hatching poultry eggs and caring for premature or sick infants, but a new and important application has recently emerged, namely, the cultivation and manipulation of microorganisms for medical treatment and research. This article will focus on laboratory (medical) incubators.

The first incubators were used in ancient China and Egypt, where they consisted of fire-heated rooms in which fertilized chicken eggs were placed to hatch, thereby freeing the hens to continue laying eggs. Later, wood stoves and alcohol lamps were used to heat incubators. Today, poultry incubators are large rooms, electrically heated to maintain temperatures between 99.5 and 100 degrees Fahrenheit (37.5 and 37.8 degrees Celsius). Fans are used to circulate the heated air evenly over the eggs, and the room's humidity is set at about 60 percent to minimize the evaporation of water from the eggs. In addition, outside air is pumped into the incubator to maintain a constant oxygen level of 21 percent, which is normal for fresh air. As many as 100,000 eggs may be nurtured in a large commercial incubator at one time, and all are rotated a minimum of 8 times a day throughout the 21-day incubation period.

During the late nineteenth century, physicians began to use incubators to help save the lives of babies born after a gestation period of less than 37 weeks (an optimal human pregnancy lasts 280 days, or 40 weeks). The first infant incubator, heated by kerosene lamps, appeared in 1884 at a Paris women's hospital.

In 1933, American Julius H. Hess designed an electrically heated infant incubator (most are still electrically heated today). Modern baby incubators resemble cribs, save that they are enclosed. Usually, the covers are transparent so that medical personnel can observe babies continually. In addition, many incubators are made with side wall apertures into which long-armed rubber gloves can be fitted, enabling nurses to care for the babies without removing them. The temperature is usually maintained at between 88 and 90 degrees Fahrenheit (31 to 32 degrees Celsius). Entering air is passed through a HEPA (high efficiency purified air) filter, which cleans and humidifies it, and the oxygen level within the chamber is adjusted to meet the particular needs of each infant. Incubators in neonatal units, centers that specialize in caring for premature infants, are frequently equipped with electronic devices for monitoring the infant's temperature and the amount of oxygen in its blood.

Laboratory (medical) incubators were first utilized during the twentieth century, when doctors realized that they could be could be used to identify pathogens (disease-causing bacteria) in patients' bodily fluids and thus diagnose their disorders more accurately. After a sample has been obtained, it is transferred to a Petri dish, flask, or some other sterile container and placed in a rack inside the incubator. To promote pathogenic growth, the air inside the chamber is humidified and heated to body temperature (98.6 degrees Fahrenheit or 37 degrees Celsius). In addition, these incubators provide the amount of atmospheric carbon dioxide or nitrogen necessary for the cell's growth. As this carefully conditioned air circulates around it, the microorganism multiplies, enabling easier and more certain identification.

A related use of incubators is tissue culture, a research technique in which clinicians extract tissue fragments from plants or animals, place these explants in an incubator, and monitor their subsequent growth. The temperature within the incubator is maintained at or near that of the organism from which the explant was derived. Observing explants in incubators gives scientists insight into the operation and interaction of particular cells; for example, it has enabled them to understand cancerous cells and to develop vaccines for polio, influenza, measles, and mumps. In addition, tissue culture has allowed researchers to detect disorders stemming from the lack of particular enzymes.

Incubators are also used in genetic engineering, an extension of tissue culturing in which scientists manipulate the genetic materials in explants, sometimes combining DNA from discrete sources to create new organisms. While such applications as sperm banks, cloning, and eugenics trouble many contemporary observers, genetic material has already been manipulated to measurable positive effect—to make insulin and other biologically essential proteins, for example. Genetic engineering can also improve the nutritional content of many fruits and vegetables and can increase the resistance of various crops to disease. It is in the field of bio-technology that incubators' greatest potential lies.

Raw Materials

Three main types of materials are necessary to manufacture an incubator. The first is stainless steel sheet metal of a common grade, usually .02 to .04 inch (.05 to .1 centimeter) thick. Stainless steel is used because it resists rust and corrosion that might be caused by both naturally occurring environmental agents and by whatever is placed inside the unit. The next category of necessary components includes items purchased from outside suppliers: nuts, screws, insulation, motors, fans, and other miscellaneous items. The third type of necessary material is the electronics package, whose complexity will depend upon the sophistication of the unit in question. Such a package may have simple on/off switches with analog temperature control or a state-of-the-art microprocessor that can be programmed to maintain different temperatures for varying intervals, or to operate various internal light systems.


Like standard refrigerators, incubators are measured in terms of the chamber's volume, which ranges from 5 to 10 cubic feet (1.5 to 3 cubic meters) for countertop models and from 18 to 33 cubic feet (5.5 to 10 cubic meters) for free-standing models.

The sheet metal is used to make two box configurations, an inner chamber and the case that encloses it. Insulation (if the chamber is heated electrically) or a water-jacket (if it is water-heated) surrounds the chamber, and the case supports it, the controls, and the doors. To prevent contamination and avoid fungal or bacterial growth, the chamber must be hermetically sealed, or rendered airtight, as must any apertures built into its walls. A glass door that allows scientists to observe the chamber's contents without disturbing them fits against the chamber's gasket, which helps to keep the incubator airtight. A steel door, solid and insulated, closes over the glass door.

Two types of heat sources are used: electrical heaters that use fans to circulate the warmth they generate, and hot water jackets. In the former design, the inner chamber has an electrical heater mounted on an inside wall and covered by a perforated protective panel. Mounted in the chamber wall just above the heater is a fan whose motor extends through the chamber wall into the control area of the case and whose blades face inward. Other manufacturers heat the chamber by surrounding it with a water-filled jacket.

The dry-wall heater offer several advantages over the water-jacket. First, the former can change temperature within the chamber more quickly. Also, electrically heated units can be thermally decontaminated because the wall heaters not only warm the chamber more quickly but also heat it to higher temperatures (a unit is considered contaminant-free after its chamber temperature has been raised to 212 degrees Fahrenheit or 100 degrees Celsius or above). Water jackets pose another problem wall heaters don't: because they are pressurized, they can develop leaks.

Humidity is generated by heating a small copper bowl that contains limited amounts of purified water; the resulting steam can be introduced into the chamber by means of a control valve. Interior lighting may also be used. Fluorescent and UV (ultra-violet)

The largest components in a laboratory incubator are made of stainless steel sheet metal that is sheared, perforated, and bent to the proper shape. The pieces are joined together by screws, spot welding, or arc welding. Near the end of the assembly process, either a water jacket or insulation is inserted into the chamber.
The largest components in a laboratory incubator are made of stainless steel sheet metal that is sheared, perforated, and bent to the proper shape. The pieces are joined together by screws, spot welding, or arc welding. Near the end of the assembly process, either a water jacket or insulation is inserted into the chamber.
lamps can be installed separately or in combination. To adjust temperature, humidity, lights, ventilation, and any other special features, more sophisticated incubators feature control panels on their outer case. However, if the unit is a relatively simple one, it will provide only basic on/off switches with simple analog temperature controls. Inside the chamber, a thermostat or thermocouple is strategically placed so that it can be viewed without difficulty from the outside.

The Manufacturing

Cutting, perforating, and bending
the sheet metal

Assembling the cabinets

Painting the incubator

Insulating or jacketing the chamber

Assembling the control panel

Final assembly, testing, and cleaning

Quality Control

No quality standards are accepted by the entire incubator manufacturing industry. Some areas of the country may require UL (Underwriters Laboratory) Electrical Approval, but those standards apply only to the electro-mechanical devices being used. During the sheet metal work, manufacturers utilize in-house inspection processes that can vary widely, from formal first-piece inspection to random lot sampling inspection. Some companies may keep records of their findings, while others do not. Almost without exception, manufacturers do performance-level testing before shipment as described above.

The Future

While hospitals will always need neonatal incubators, the bio-technological industry is where the growth market lies for this product. Growth chamber type incubators will need to control temperature and relative humidity to more precise settings, as microbiologists and researchers investigate new ways to improve our health and well-being.

Where To Learn More


Coyne, Gary. The Laboratory Handbook of Materials, Equipment, and Technique. Prentice Hall, 1991.

Frank Sokolo

User Contributions:

attique sajid
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Aug 1, 2006 @ 11:23 pm
this article was of great help for us to understand the design of a neonatal incubator.can you please put more light on temperature and humidity control circuits used in neonatal incubators.for your information , we are developing a neonatal incubator as final year project of BE electronics.thanking in anticipation.

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